Transcription of transposable element Activator (Ac) of Zea mays L
نویسندگان
چکیده
منابع مشابه
The Mutator-Related Cy Transposable Element of Zea Mays L. Behaves as a near-Mendelian Factor.
The bz-rcy allele arose in a single gamete of the TEL (transposable-element laden) population, when the rcy receptor element inserted into the Bronze1 locus. This newly arisen receptor allele conditions a stable bronze kernel phenotype in the absence of the independently segregating regulatory element, Cy. In the presence of Cy, bz-rcy conditions fully colored spots on a bronze background. The ...
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Activator/Dissociation (Ac/Ds) transposable elements from maize are widely used as insertional mutagenesis and gene isolation tools in plants and more recently also in medaka and zebrafish. They are particularly valuable for plant species that are transformation-recalcitrant and have long generation cycles or large genomes with low gene densities. Ac/Ds transposition frequencies vary widely, ho...
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Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...
متن کاملFrequent loss of the En transposable element after excision and its relation to chromosome replication in maize (Zea mays L.).
A model of En transposition during chromosome replication is presented following a study of somatic events associated with the transposition of En in the endosperm tissue of the maize kernel. Two supporting assays, the excision and the postexcision events, were used in following these events. The excision of the En transposon has been monitored in the starch-producing endosperm tissue by using ...
متن کاملSpontaneous germinal activation of quiescent Uq transposable elements in Zea mays L.
The spontaneous germinal activation of quiescent Uq transposable elements is reported. Thirty-nine spotted exceptions were observed at a rate of about 2 x 10(-4) from 687 otherwise colorless ears produced from the cross of a-ruq/a-ruq (colorless or occasionally sectored) X an a-ruq tester (colorless). All exceptions had spotting patterns distinct from the pattern of our original standard Uq (Uq...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1987
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1987.tb02400.x